CD3 anticorps

N° du produit ABIN457409

L’anticorps Souris Monoclonal anti-CD3 a été validé pour FACS et IHC (fro). Il convient pour détecter CD3 dans des échantillons de Humain. Il y a 7+ publications disponibles.

Aperçu rapide pour CD3 anticorps (ABIN457409)

Antigène: CD3

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OKT3

Détail du produit anti-CD3 anticorps

Fonction: Anti-Hu CD3 Purified

Specificité: The mouse monoclonal antibody OKT3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: human T cells

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD3 (ABIN457409)

Li, Wong, Yi, Wong, Zhou, Liu et al.: "Matrine induces cell anergy in human Jurkat T cells through modulation of mitogen-activated protein kinases and nuclear factor of activated T-cells signaling with concomitant up-regulation of ..." dans: Biological & pharmaceutical bulletin, Vol. 33, Issue 1, pp. 40-6, (2010) (PubMed).

Quintana, Schwindling, Wenning, Becherer, Rettig, Schwarz, Hoth: "T cell activation requires mitochondrial translocation to the immunological synapse." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14418-23, (2007) (PubMed).

Trushin, Bren, Asin, Pennington, Paya, Badley: "Human immunodeficiency virus reactivation by phorbol esters or T-cell receptor ligation requires both PKCalpha and PKCtheta." dans: Journal of virology, Vol. 79, Issue 15, pp. 9821-30, (2005) (PubMed).

Undale, van den Elsen, Celis: "Antigen-independent acquisition of MHC class II molecules by human T lymphocytes." dans: International immunology, Vol. 16, Issue 10, pp. 1523-33, (2004) (PubMed).

Lang, Handgretinger, Niethammer, Schlegel, Schumm, Greil, Bader, Engel, Scheel-Walter, Eyrich, Klingebiel: "Transplantation of highly purified CD34+ progenitor cells from unrelated donors in pediatric leukemia." dans: Blood, Vol. 101, Issue 4, pp. 1630-6, (2003) (PubMed).

Schlegel, Eyrich, Bader, Handgretinger, Lang, Niethammer, Klingebiel: "OKT-3-based reconditioning regimen for early graft failure in HLA-non-identical stem cell transplants." dans: British journal of haematology, Vol. 111, Issue 2, pp. 668-73, (2001) (PubMed).

Smith: "Ten years of Orthoclone OKT3 (muromonab-CD3): a review." dans: Journal of transplant coordination : official publication of the North American Transplant Coordinators Organization (NATCO), Vol. 6, Issue 3, pp. 109-19; quiz 120-1, (1997) (PubMed).

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Sujet: CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway