alpha 2 Macroglobulin anticorps

N° du produit ABIN457943

L’anticorps Chèvre Polyclonal anti-alpha 2 Macroglobulin a été validé pour IEP. Il convient pour détecter alpha 2 Macroglobulin dans des échantillons de Humain.

Aperçu rapide pour alpha 2 Macroglobulin anticorps (ABIN457943)

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha 2 Macroglobulin est non-conjugé

Application: Immunoelectrophoresis (IEP)

Détail du produit anti-alpha 2 Macroglobulin anticorps

Specificité: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has been tested in double radial immunodiffusion against several ser of other species with the following results: bovine + dog ± cat + goat - monkey ++ rabbit + mouse - rat -

Attributs du produit: Precipitating polyclonal goat antiserum to human alpha-2 macroglobulin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Alpha-2 macroglobulin(clotting factor I) is the major protein in the alpha-2 region of human serum after separation by immunoelectrophoresis. The concentration in normal plasma is about 2.5 mg/ml. It is a large polymer and a strong antigen. It forms about one third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gelfiltration and preparative starch block electrophoresis. In the purification the activation of kallikrein is avoided, since it forms an irreversible bond with alpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single or double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of alpha-2 macroglobulin in human plasma or other body fluids or to determine its concentration. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays, but this does exclude such use if proper controls are included.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre 1:64 when tested against pooled normal human plasma in agar-block immunodiffusion titration. No foreign proteins ad

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Agent conservateur: Without preservative

Stock: 4 °C

Détails sur alpha 2 Macroglobulin

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Autre désignation: alpha-2 Macroglobulin

Sujet: The reactivity of the antiserum is restricted to human alpha-2 macroglobulin. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-2 macroglobulin. No reaction is obtained with any other plasma protein components or serum

Pathways: Lipid Metabolism