Complement Factor B anticorps

N° du produit ABIN457969

Cet anticorps Chèvre Polyclonal détecte spécifiquement Complement Factor B dans IP. Il présente une réactivité envers Humain.

Aperçu rapide pour Complement Factor B anticorps (ABIN457969)

Antigène: Complement Factor B (CFB)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Complement Factor B est non-conjugé

Application: Immunoprecipitation (IP)

Détail du produit anti-Complement Factor B anticorps

Specificité: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a weak reaction with mouse has been observed.

Attributs du produit: Precipitating polyclonal goat antiserum to human properdin factor B

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Properdin factor B is a heat-labile serum protein active in the alternative complement activation pathway. It is binding to the C3b component of complement. The protein has a molecular weight of 92,000 and its mean concentration in human plasma is 200 µg/ml. It has been isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques for the identification and quantitative determination of properdin factor B in body fluids. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Preciptin titre not less than 1:8 when tested against normal human serum in agar block immunodiffusion tit

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Détails sur Complement Factor B

Antigène: Complement Factor B (CFB)

Autre désignation: Properdin Factor B

Sujet: Tested in immunoelectrophoresis against human EDTA plasma a single precipitin line is obtained in the beta-2 region. The antiserum does not react with any other protein component of human serum or plasma

Pathways: Système du Complément, Proton Transport, Ribonucleoside Biosynthetic Process