Complement C3b anticorps

N° du produit ABIN458141

L’anticorps Lapin Polyclonal anti-Complement C3b a été validé pour IEP. Il convient pour détecter Complement C3b dans des échantillons de Humain.

Aperçu rapide pour Complement C3b anticorps (ABIN458141)

Antigène: Complement C3b (C3b) (Complement C3 beta Chain (C3b))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Complement C3b est non-conjugé

Application: Immunoelectrophoresis (IEP)

Détail du produit anti-Complement C3b anticorps

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Precipitating polyclonal rabbit antiserum to C3b subunit of human complement C3

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: C3b (C3c+C3d) has a molecular weight of 170,000. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of complement C3b or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:80 when tested against normal human plasma in agar-block immunodiffusi

Buffer: Delipidated, heat inactivated, lyophilized, stable whole serum.

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Détails sur Complement C3b

Antigène: Complement C3b (C3b) (Complement C3 beta Chain (C3b))

Autre désignation: C3b Subunit of Complement C3

Sujet: In immunoelectrophoresis and radial immunodiffusion, using various antiserum concen-trations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of human serum or plasma

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling