Cholesterol Esterase anticorps (Biotin)

N° du produit ABIN459017

L’anticorps anti-Cholesterol Esterase Polyclonal Lapin est utilisé pour la détection de Cholesterol Esterase dans des échantillons de Candida sp.. Il a été validé pour WB, ELISA, ICC, IHC (p) et DB.

Aperçu rapide pour Cholesterol Esterase anticorps (Biotin) (ABIN459017)

Antigène: Cholesterol Esterase (CEL)

Reactivité: Candida sp.

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cholesterol Esterase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Détail du produit anti-Cholesterol Esterase anticorps (Biotin)

Fonction: Rabbit anti Candida cylindracea Cholesterol esterase, conjugated with Biotin

Specificité: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to cholesterol esterase from Candida cylindracea.

Réactivité croisée (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Purification: Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogène: Cholesterol esterase isolated and purified from Candida cylindracea. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détails sur Cholesterol Esterase

Antigène: Cholesterol Esterase (CEL)

Autre désignation: Cholesterol Esterase

UniProt: P32947

Pathways: Lipid Metabolism