FLIP anticorps (AA 191-209)

N° du produit ABIN461877

Cet anticorps anti-FLIP est un anticorps Lapin Polyclonal détectant FLIP dans WB, ELISA, IHC, ICC et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour FLIP anticorps (AA 191-209) (ABIN461877)

Antigène: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FLIP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-FLIP anticorps

Épitope: AA 191-209

Marque: IHC-plus™

Specificité: Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.

Purification: Immunoaffinity purified

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, ICC, IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kD and 25 kD bands can be detected. The antibody recognizes human and murine FLIPg and FLIPd but not FLIPa and FLIPb.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur FLIP

Antigène: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Autre désignation: CFLAR / FLIP

Sujet: Name/Gene ID: CFLAR
Subfamily: Cysteine C14
Family: Apoptosis

Synonyms: CFLAR, C-FLIPR, C-FLIPS, C-FLIP, CASP8AP1, Cflip, CLARP, CASH, Caspase homolog, Caspase-eight-related protein, Flice inhibitory protein, I-FLICE, Inhibitor of FLICE, FLAME, FLAME1, MRIT, Usurpin beta, C-FLIPL, Casper, FLAME-1, FLIP, Usurpin

ID gène: 8837

UniProt: O15519

Pathways: Apoptose, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development