CFLAR anticorps (CASP8 and FADD-Like Apoptosis Regulator) (AA 191-209)

Details for Product anti-CFLAR Antibody No. ABIN461877
Antigène
Épitope
AA 191-209
32
26
20
16
15
10
8
7
6
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris
215
96
72
5
2
2
1
1
Hôte
Lapin
172
52
5
Clonalité
Polyclonal
Conjugué
Cet anticorp CFLAR est non-conjugé
8
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
185
76
68
34
30
29
26
20
12
11
3
2
2
1
Options
Marque IHC-plus™
Isotype IgG
Specificité Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.
Purification Immunoaffinity purified
Antigène
Autre désignation CFLAR / FLIP (CFLAR Antibody Extrait)
Sujet Name/Gene ID: CFLAR
Subfamily: Cysteine C14
Family: Apoptosis

Synonyms: CFLAR, C-FLIPR, C-FLIPS, C-FLIP, CASP8AP1, Cflip, CLARP, CASH, Caspase homolog, Caspase-eight-related protein, Flice inhibitory protein, I-FLICE, Inhibitor of FLICE, FLAME, FLAME1, MRIT, Usurpin beta, C-FLIPL, Casper, FLAME-1, FLIP, Usurpin
ID gène 8837
UniProt O15519
Pathways Apoptose, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Indications d'application Approved: ELISA, ICC, IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kD and 25 kD bands can be detected. The antibody recognizes human and murine FLIPg and FLIPd but not FLIPa and FLIPb.
Commentaires

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, 0.02 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation avoid freeze thaw cycles. Store undiluted.
Stock 4 °C,-20 °C
Stockage commentaire Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Date de péremption 12 months
Images (Fournisseur)
Image no. 1 for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209) antibody (ABIN461877) Human Pancreas (formalin-fixed, paraffin-embedded) stained with CFLAR antibody ABIN46...
Image no. 2 for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209) antibody (ABIN461877) Human Lung (formalin-fixed, paraffin-embedded) stained with CFLAR antibody ABIN461877...
Image no. 3 for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209) antibody (ABIN461877) Human Placenta (formalin-fixed, paraffin-embedded) stained with CFLAR antibody ABIN46...
Image no. 4 for anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209) antibody (ABIN461877) Anti-CFLAR / FLIP antibody IHC of human lung. Immunohistochemistry of formalin-fixed,...