TDP1 anticorps (AA 1-298)

N° du produit ABIN462272

L’anticorps Souris Monoclonal anti-TDP1 a été validé pour WB, ELISA, IHC et IHC (p). Il convient pour détecter TDP1 dans des échantillons de Humain.

Aperçu rapide pour TDP1 anticorps (AA 1-298) (ABIN462272)

Antigène: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TDP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT1F2

Détail du produit anti-TDP1 anticorps

Épitope: AA 1-298

Specificité: Human TDP1 (1-298aa)

Purification: Protein G purified

Immunogène: Recombinant human TDP1 (1-298aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TDP1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Détails sur TDP1

Antigène: TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))

Autre désignation: TDP1

Sujet: Name/Gene ID: TDP1

Synonyms: TDP1, Tyr-DNA phosphodiesterase 1, SCAN1

ID gène: 55775

UniProt: Q9NUW8