Cytochrome C anticorps

N° du produit ABIN462359

L’anticorps Souris Monoclonal anti-Cytochrome C a été validé pour WB, IHC et IHC (p). Il convient pour détecter Cytochrome C dans des échantillons de Humain, Rat, Souris, Cheval, Lapin, Porc, Boeuf (Vache), Mouton, Singe, Hamster et Cobaye.

Aperçu rapide pour Cytochrome C anticorps (ABIN462359)

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivité: Humain, Rat, Souris, Cheval, Lapin, Porc, Boeuf (Vache), Mouton, Singe, Hamster, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytochrome C est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7H8-2C12

Détail du produit anti-Cytochrome C anticorps

Specificité: Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c in applications such as Western blot, it does not recognize the native protein. The epitope of this antibody was mapped to a region within amino acids 93-104 of horse Cytochrome.

Purification: Protein G purified

Immunogène: Horse cytochrome c.

Type of Immunogen: Protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Horse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 0.09 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur Cytochrome C

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: CYCS / Cytochrome c

Sujet: Name/Gene ID: CYCS

Synonyms: CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, somatic

ID gène: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity