CHRNA7 anticorps

N° du produit ABIN462402

Cet anticorps anti-CHRNA7 Polyclonal Chèvre (ABIN462402) détecte spécifiquement CHRNA7 dans IHC, ELISA et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Boeuf (Vache), Poulet, Singe, Chien, Porc et Cheval.

Aperçu rapide pour CHRNA7 anticorps (ABIN462402)

Antigène: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Reactivité: Humain, Boeuf (Vache), Poulet, Singe, Chien, Porc, Cheval

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CHRNA7 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CHRNA7 anticorps

Specificité: Human CHRNA7. This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Horse, Pig, Opossum, Chicken (100%) Mouse, Rat, Hamster, Rabbit (94%) Marmoset, Xenopus, Zebrafish (88%) Bat, Platypus (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide KRPGEDKVRPACQHKQ from an internal region of human CHRNA7 (NP_000737.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Horse, Pig, Opossum, Chicken (100%), Mouse, Rat, Hamster, Rabbit (94%), Marmoset, Xenopus, Zebrafish (88%), Bat, Platypus (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (3 - 6 μg/mL), Peptide-ELISA

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur CHRNA7

Antigène: CHRNA7 (Cholinergic Receptor, Nicotinic, alpha 7 (Neuronal) (CHRNA7))

Autre désignation: CHRNA7

Sujet: Name/Gene ID: CHRNA7
Subfamily: Nicotinic acetylcholine receptor
Family: Ion Channel

Synonyms: CHRNA7, AChR-alpha7, Acra 7, CHRNA7-2, NAChR alpha 7, NACHRA7

ID gène: 1139

NCBI Accession: NP_000737

UniProt: P36544

Pathways: Synaptic Membrane