CAMK2B anticorps

N° du produit ABIN462429

Cet anticorps anti-CAMK2B est un anticorps Lapin Polyclonal détectant CAMK2B dans WB, IHC et IHC (p). Adapté pour Humain, Rat, Souris, Boeuf (Vache), Cheval, Lapin, Poisson zèbre (Danio rerio), Chien, Singe, Xenopus laevis, Poulet et Porc.

Aperçu rapide pour CAMK2B anticorps (ABIN462429)

Antigène: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Cheval, Lapin, Poisson zèbre (Danio rerio), Chien, Singe, Xenopus laevis, Poulet, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CAMK2B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CAMK2B anticorps

Marque: IHC-plus™

Specificité: Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%) Orangutan (93%).

Purification: Immunoaffinity purified

Immunogène: A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%), Orangutan (93%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: BBS,  pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)

Stock: -20 °C

Stockage commentaire: Store at -20°C.

Détails sur CAMK2B

Antigène: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Autre désignation: CAMK2 / CAMKII

Sujet: Name/Gene ID: CAMK2

Synonyms: CAMK2

Pathways: Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity