Interferon gamma anticorps (Biotin)

N° du produit ABIN464925

L’anticorps Chèvre Polyclonal anti-Interferon gamma a été validé pour WB, ELISA et Neut. Il convient pour détecter Interferon gamma dans des échantillons de Rat.

Aperçu rapide pour Interferon gamma anticorps (Biotin) (ABIN464925)

Antigène: Interferon gamma (IFNG)

Reactivité: Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Interferon gamma est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-Interferon gamma anticorps (Biotin)

Specificité: Rat IFN-gamma

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Rat IFN-gamma.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat IFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma. To detect Rat IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IFN-gamma (LS-C104323) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma. Western Blot: To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur Interferon gamma

Antigène: Interferon gamma (IFNG)

Autre désignation: IFN Gamma / Interferon Gamma

Sujet: Name/Gene ID: IFNG
Family: Interferon

Synonyms: IFNG, IFG, Immune interferon, IFI, Interferon gamma, IFN-gamma, Interferon, gamma

ID gène: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy