IL-3 anticorps (Biotin)

N° du produit ABIN465198

L’anticorps Lapin Polyclonal anti-IL-3 a été validé pour WB, ELISA et Neut. Il convient pour détecter IL-3 dans des échantillons de Humain.

Aperçu rapide pour IL-3 anticorps (Biotin) (ABIN465198)

Antigène: IL-3 (Interleukin 3 (IL-3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-3 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-IL-3 anticorps (Biotin)

Specificité: Human IL-3

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human IL-3.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-3. To detect hIL-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-3 (LS-C104470) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-3. Western Blot: To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur IL-3

Antigène: IL-3 (Interleukin 3 (IL-3))

Autre désignation: IL3

Sujet: Name/Gene ID: IL3
Family: Hormone

Synonyms: IL3, Hematopoietic growth factor, IL-3, Mast-cell growth factor, MULTI-CSF, P-cell-stimulating factor, Interleukin-3, Mast cell growth factor, MCGF, P-cell stimulating factor

ID gène: 3562

UniProt: P08700

Pathways: Signalistation JAK/STAT, Regulation of Carbohydrate Metabolic Process, Autophagy