FGF1 anticorps (Biotin)
Aperçu rapide pour FGF1 anticorps (Biotin) (ABIN465423)
Antigène
Voir toutes FGF1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Human FGF-acidic
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Purification
- Immunoaffinity purified
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Immunogène
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E.coli derived recombinant Human FGF-acidic.
Type of Immunogen: Cell extract
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Indications d'application
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hFGF-acidic by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-acidic. To detect hFGF-acidic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human FGF-acidic (LS-B8222) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Sterile PBS containing 0.1 % BSA
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Conseil sur la manipulation
- Avoid freeze/thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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Autre désignation
- FGF1
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Sujet
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Name/Gene ID: FGF1
Family: HBGF
Synonyms: FGF1, AFGF, Endothelial cell growth factor, Fibroblast growth factor 1, FGFA, ECGFB, HBGF-1, GLIO703, ECGF, ECGF-beta, ECGFA, FGF-1, FGF-alpha, HBGF1 -
ID gène
- 2246
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UniProt
- P05230
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Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Antigène
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