FZD3 anticorps (Cysteine-Rich Domain, Extracellular) (Biotin)

N° du produit ABIN470848

Cet anticorps Rat Monoclonal détecte spécifiquement FZD3 dans WB, IHC, FACS, ICC et IHC (fro). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour FZD3 anticorps (Cysteine-Rich Domain, Extracellular) (Biotin) (ABIN470848)

Antigène: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp FZD3 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-FZD3 anticorps (Biotin)

Épitope: Cysteine-Rich Domain, Extracellular

Specificité: Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 and -8. Species cross-reactivity: Human.

Purification: Protein G purified

Immunogène: NS0-derived rmFrizzled-3 extracellular CRD.

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, ICC, IHC, IHC-Fr, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Detected Frizzled-3 by flow cytometry and immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Immunocytochemistry and flow cytometry experiments on human HEK 293 cells demonstrate that this antibody cross-reacts with human Frizzled-3. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit for rmFrizzled-3 is ~0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute this antibody to 0.2 mg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: Use with the appropriate secondary reagents at 8 μg/mL to detect Frizzled-3 in cells or frozen mouse embryos. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 1 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, BSA

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur FZD3

Antigène: FZD3 (Frizzled Family Receptor 3 (FZD3))

Autre désignation: FZD3 / Frizzled 3

Sujet: Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3

ID gène: 7976

UniProt: Q9NPG1

Pathways: Signalisation WNT, Tube Formation