FZD1 anticorps (Extracellular Domain) (Biotin)

N° du produit ABIN478743

Cet anticorps anti-FZD1 est un anticorps Rat Monoclonal détectant FZD1 dans WB, ICC et FACS. Adapté pour Humain et Souris.

Aperçu rapide pour FZD1 anticorps (Extracellular Domain) (Biotin) (ABIN478743)

Antigène: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp FZD1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-FZD1 anticorps (Biotin)

Épitope: Extracellular Domain

Specificité: Recognizes mouse Frizzled-1 extracellular domain. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry. Species cross-reactivity: Human.

Purification: Protein G purified

Immunogène: NS0-derived rmFrizzled-1 extracellular domain.

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, ICC, WB

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 10 μL of this solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 mL of a 25 mg/mL stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome. Immunocytochemistry: Used with appropriate secondary reagents at 5-10 μg/mL in 4 % . paraformaldehyde-fixed cells. Western Blot: Use at 1-2 μg/mL with appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, BSA.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur FZD1

Antigène: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Autre désignation: FZD1 / Frizzled 1

Sujet: Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1

ID gène: 8321

UniProt: Q9UP38

Pathways: Signalisation WNT, Asymmetric Protein Localization