FZD1 anticorps (Extracellular Domain) (Biotin)

N° du produit ABIN478743

Détail du produit anti-FZD1 anticorps (Biotin)

Antigène: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Épitope: Extracellular Domain

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp FZD1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificité: Recognizes mouse Frizzled-1 extracellular domain. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry. Species cross-reactivity: Human.

Purification: Protein G purified

Immunogène: NS0-derived rmFrizzled-1 extracellular domain.

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, ICC, WB

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 10 μL of this solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 mL of a 25 mg/mL stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome. Immunocytochemistry: Used with appropriate secondary reagents at 5-10 μg/mL in 4 % . paraformaldehyde-fixed cells. Western Blot: Use at 1-2 μg/mL with appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, BSA.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur FZD1

Antigène: FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

Autre désignation: FZD1 / Frizzled 1 (Fzd1 Produits)

Synonymes: anticorps CG17697, anticorps CG3646, anticorps DFZ1, anticorps DFz, anticorps DFz1, anticorps Dfz1, anticorps Dm Fz1, anticorps Dmel\\CG17697, anticorps FZ, anticorps Frz, anticorps Fz, anticorps Fz1, anticorps frz, anticorps fz1, anticorps fz[[1]], anticorps Fz-1, anticorps cFz-1, anticorps Xfrizzled-1, anticorps frizzled1, anticorps frz-1, anticorps frz1, anticorps fzd1-A, anticorps AW227548, anticorps FZ-1, anticorps frizzled, anticorps frizzled class receptor 1, anticorps frizzled class receptor 1 L homeolog, anticorps fz, anticorps FZD1, anticorps Fzd1, anticorps fzd1.L

Sujet: Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1

ID gène: 8321

UniProt: Q9UP38

Pathways: Signalisation WNT, Asymmetric Protein Localization