LIME anticorps (AA 86-202)

N° du produit ABIN487426

Cet anticorps Souris Monoclonal détecte spécifiquement LIME dans WB et IP. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour LIME anticorps (AA 86-202) (ABIN487426)

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LIME est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: MLIME-05

Détail du produit anti-LIME anticorps

Épitope: AA 86-202

Specificité: This antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.

Réactivité croisée (Details): Species reactivity (tested):Mouse

Attributs du produit: Synonyms: LIME1, LIME, LIME-1, Lck-interacting membrane protein, Lck-interacting molecule,Lck-interacting transmembrane adapter 1

Purification: Protein A affinity chromatography

Immunogène: Recombinant protein corresponding to amino acids 86-202 of mouse LIME

Isotype: IgG2a

Information d'application

Indications d'application: Immunoprecipitation (use murine splenic T cells as positive control). Western blot: 1-5 μg/mL (reducing conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg /ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur LIME

Antigène: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Autre désignation: LIME1

Sujet: LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

ID gène: 72699

UniProt: Q9EQR5