Adenosine A2a Receptor anticorps

N° du produit ABIN487435

Cet anticorps Souris Monoclonal détecte spécifiquement Adenosine A2a Receptor dans WB, FACS et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Chien, Lapin et Cobaye.

Aperçu rapide pour Adenosine A2a Receptor anticorps (ABIN487435)

Antigène: Adenosine A2a Receptor (ADORA2A)

Reactivité: Humain, Souris, Rat, Chien, Lapin, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Adenosine A2a Receptor est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: N-n

Détail du produit anti-Adenosine A2a Receptor anticorps

Specificité: This antibody recognizes Human Adenosine Receptor A2A at ~43 kDa. Does not recognize Adenosine Receptor A2A in knockout mice.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Guinea Pig and Rabbit.
Species reactivity (tested):Human.

Attributs du produit: Synonyms: ADORA2A, ADORA2

Purification: Protein G Chromatography

Immunogène: 6His-tagged full-length Human recombinant A2A receptor. Genename: ADORA2ARemarks: Epitope mapped to SQPLPGER in the third intracellular loop.

Isotype: IgG2a

Information d'application

Indications d'application: Western blot: 1/1000-1/5000. Flow Cytometry. Immunohistochemistry (Paraffin Sections): 5 μg/mL. Immunohistochemistry (4 % Paraformaldehyde): 1 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: 0.01 M Tris-Glycine, pH 7.4, 0.11 M sodium chloride, 0.05 % sodium azide.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Adenosine A2a Receptor

Antigène: Adenosine A2a Receptor (ADORA2A)

Autre désignation: Adenosine Receptor A2a

Sujet: The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.Synonyms: ADORA2, ADORA2A

ID gène: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints