AGO1 anticorps (N-Term)

N° du produit ABIN488536

Cet anticorps Lapin Polyclonal détecte spécifiquement AGO1 dans WB. Il présente une réactivité envers Arabidopsis thaliana et Nicotiana benthamiana et a été mentionné dans 1 publication.

Aperçu rapide pour AGO1 anticorps (N-Term) (ABIN488536)

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivité: Arabidopsis thaliana, Nicotiana benthamiana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-AGO1 anticorps

Épitope: N-Term

Specificité: Antibody binds microRNA and tasiRNAs, preference for 21ntmiRNAs with 5'U

Réactivité croisée (Details): Not reactive in: Chlamydomonas reinhardtii

Homologie: Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 116.4 / 130 kDa
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

Purification: serum

Immunogène: N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410

Information d'application

Indications d'application: 1: 5000 - 1: 10 000 (WB)

Commentaires:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Conseil sur la manipulation: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-AGO1 (ABIN488536)

Speth, Willing, Rausch, Schneeberger, Laubinger: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." dans: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).

Détails sur AGO1

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Autre désignation: AGO1

Sujet: AGI Code: At1g48410
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Poids moléculaire: expected: 116.4 kDa, apparent: 130 kDa

UniProt: O04379

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization