HNRNPF anticorps (N-Term)

N° du produit ABIN4886622

Cet anticorps anti-HNRNPF Polyclonal Lapin (ABIN4886622) détecte spécifiquement HNRNPF dans WB, IF, IHC, ICC, IHC (fro) et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour HNRNPF anticorps (N-Term) (ABIN4886622)

Antigène: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HNRNPF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Détail du produit anti-HNRNPF anticorps

Épitope: N-Term

Fonction: Anti-HnRNP F/HNRNPF Antibody

Séquence: SVEDVQNFLS DCTIHDGAAG VHFIYTREGR Q

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: different from the related mouse,rat sequences by two amino acids.

Attributs du produit: Anti-HnRNP F/HNRNPF Antibody. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur HNRNPF

Antigène: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Autre désignation: HNRNPF

Sujet:

Background: Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gene Full Name: heterogeneous nuclear ribonucleoprotein F

Poids moléculaire: 46 kDa

ID gène: 3185

UniProt: P52597