CD3 anticorps

N° du produit ABIN490154

L’anticorps Souris Monoclonal anti-CD3 a été validé pour FACS, IP, IHC, IHC (fro), WB et ICC. Il convient pour détecter CD3 dans des échantillons de Humain et Souris.

Aperçu rapide pour CD3 anticorps (ABIN490154)

Antigène: CD3

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunocytochemistry (ICC)

Clone: APA1-1

Détail du produit anti-CD3 anticorps

Specificité: The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Purification: Protein A purified

Immunogène: Purified human CD3 proteins isolated from thymus.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo, ICC, IHC, IHC-Fr, IP, WB

Usage: Flow Cytometry: Positive control - Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation:. At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway