Aurora A anticorps (C-Term)

N° du produit ABIN492609

Cet anticorps anti-Aurora A Monoclonal Souris (Clone 5F11) (ABIN492609) détecte spécifiquement Aurora A dans WB, IF et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Aurora A anticorps (C-Term) (ABIN492609)

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Aurora A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 5F11

Détail du produit anti-Aurora A anticorps

Épitope: C-Term

Specificité: Recognizes Aurora-A/B (C-term).

Attributs du produit: Synonyms: Aurora kinase A, Aurora A, Aurora B, AURKA, AURKB, AURA, AURB, BTAK, STK6, ARK1, STK15,AIK, AIK2, AIM1, ARK2, STK12

Purification: Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Peptide conjugated to hemocyaninEpitope: C-terminus.

Isotype: IgG1

Information d'application

Indications d'application: Immunobotting (Western Blot): 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blotincubation buffer. Included Postitive Control: Cell lysate from untreated SW620 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive Control: Cell lysate from untreated SW620 cells. Formulation: Lyophilized Cell lysate from Serum starved SW620 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of PositiveControl correspond to ca. 20. 000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as Immunoprecipitation.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Aurora A

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Autre désignation: Aurora Kinase A

Sujet: Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2, STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.Synonyms: AIK, AIK2, AIM1, ARK1, ARK2, AURA, AURB, AURKA, AURKB, Aurora A, Aurora B, Aurora kinase A, BTAK, STK12, STK15, STK6

ID gène: 6790

UniProt: O14965

Pathways: Cycle Cellulaire, Asymmetric Protein Localization