Recombinant S100B anticorps

N° du produit ABIN4949395

L’anticorps Lapin Monoclonal anti-S100B a été validé pour WB, IF, FACS et IHC (p). Il convient pour détecter S100B dans des échantillons de Humain, Rat, Souris et Boeuf (Vache).

Aperçu rapide pour Recombinant S100B anticorps (ABIN4949395)

Antigène: S100B (S100 Calcium Binding Protein B (S100B))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp S100B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: S100B-1706R

Détail du produit anti-S100B anticorps

Purification: Protein A affinity chromatography

Immunogène: Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur S100B

Antigène: S100B (S100 Calcium Binding Protein B (S100B))

Autre désignation: S100B

Sujet: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins