CD73 anticorps (AA 520-550)

N° du produit ABIN3029822

Détail du produit anti-CD73 anticorps

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Épitope: AA 520-550

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD73 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Purified

Immunogène: A portion of amino acids 520-550 from the human protein was used as the immunogen for this CD73 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the CD73 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the CD73 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur CD73

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Autre désignation: CD73 (NT5E Produits)

Synonymes: anticorps CALJA, anticorps CD73, anticorps E5NT, anticorps NT, anticorps NT5, anticorps NTE, anticorps eN, anticorps eNT, anticorps zgc:63784, anticorps NT5E, anticorps MGC114869, anticorps PSPTO2277, anticorps 2210401F01Rik, anticorps AI447961, anticorps Nt5, anticorps 5'-nucleotidase ecto, anticorps 5'-nucleotidase, ecto (CD73), anticorps 5'-nucleotidase, anticorps 5' nucleotidase, ecto, anticorps NT5E, anticorps nt5e, anticorps nt5e.S, anticorps PSPTO_2277, anticorps CBU_1233, anticorps MCA1322, anticorps Nt5e

Sujet: Ecto-5-prime-nucleotidase/5-prime-ribonucleotide phosphohydrolase/NT5E/CD73 catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E/CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process