Cyclin D1 anticorps (AA 68-97)

N° du produit ABIN3030588

Cet anticorps anti-Cyclin D1 est un anticorps Lapin Polyclonal détectant Cyclin D1 dans WB, IHC, ELISA et IF. Adapté pour Humain et Souris.

Aperçu rapide pour Cyclin D1 anticorps (AA 68-97) (ABIN3030588)

Antigène: Cyclin D1 (CCND1)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin D1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-Cyclin D1 anticorps

Épitope: AA 68-97

Réactivité croisée (Details): Expected species reactivity: Bovine

Purification: Antigen affinity purified

Immunogène: A portion of amino acids 68-97 from the human protein was used as the immunogen for this Cyclin D1 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the Cyclin D1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur Cyclin D1

Antigène: Cyclin D1 (CCND1)

Sujet: CCND1 is the regulatory component of the Cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. CyclinD-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. [UniProt]

UniProt: P24385

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, ER-Nucleus Signaling