This phospho-HER3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1289 of human HER3/ERBB3.
ERBB3
Reactivité: Humain, Souris, Rat
ELISA, IHC (p), IF (p), IF (cc), IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Titration of the phospho-HER3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500,Western blot: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Aliquot the phospho-HER3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form.