VCP anticorps

N° du produit ABIN3029569

Cet anticorps Souris Monoclonal détecte spécifiquement VCP dans WB, ELISA et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour VCP anticorps (ABIN3029569)

Antigène: VCP (Valosin Containing Protein (VCP))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VCP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 1344CT150-163-114

Détail du produit anti-VCP anticorps

Purification: Purified

Immunogène: This VCP antibody was produced from a mouse immunized with a recombinant protein from human VCP.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Titration of the VCP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:25,Western blot: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the VCP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur VCP

Antigène: VCP (Valosin Containing Protein (VCP))

Autre désignation: VCP

Sujet: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168- dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage.

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway