AGO1 anticorps (N-Term) (Biotin)

N° du produit ABIN4966092

Cet anticorps anti-AGO1 Polyclonal Lapin conjugué à Biotin (ABIN4966092) détecte spécifiquement AGO1 dans WB. L’anticorps est réactif avec des échantillons de Arabidopsis thaliana.

Aperçu rapide pour AGO1 anticorps (N-Term) (Biotin) (ABIN4966092)

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO1 est conjugé à/à la Biotin

Application: Western Blotting (WB)

Détail du produit anti-AGO1 anticorps (Biotin)

Épitope: N-Term

Fonction: Anti-AGO1 | Argonaute 1 (40 μg, Biotin conjugated)

Réactivité croisée (Details): Arabidopsis thaliana
Not reactive in: Chlamydomonas reinhardtii

Homologie: Brassica pekinensis,Capsella rubella,Malus domestica,Pisum sativum,Ricinus communis,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogène: KLH-conugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379

Information d'application

Indications d'application: 1 : 1000 (WB)

Commentaires:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.

Conseil sur la manipulation: A preservative may be added for long time storage

Stock: 4 °C

Stockage commentaire: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Date de péremption: 12-24 months

Détails sur AGO1

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Autre désignation: argonaute 1 (AGO1)

Sujet:

Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Biotin.

Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method

Poids moléculaire: 116.4 | 130 kDa

ID gène: 841262

UniProt: O04379

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization