CDC25A anticorps

N° du produit ABIN498254

L’anticorps anti-CDC25A Polyclonal Lapin est utilisé pour la détection de CDC25A dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour CDC25A anticorps (ABIN498254)

Antigène: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC25A est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CDC25A anticorps

Specificité: This antibody detects endogenous levels of Cdc25A protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur CDC25A

Antigène: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Autre désignation: CDC25A

Sujet: The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40 % identity in their most conserved carboxy terminal sequences.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Poids moléculaire: approx. 59 kDa

ID gène: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase