PRKAG1 anticorps

N° du produit ABIN498427

Cet anticorps Lapin Polyclonal détecte spécifiquement PRKAG1 dans WB et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PRKAG1 anticorps (ABIN498427)

Antigène: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAG1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-PRKAG1 anticorps

Specificité: This antibody detects endogenous levels of AMPKgamma1/2/3 protein. (region surrounding Lys78)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PRKAG1

Antigène: PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))

Autre désignation: AMPK gamma-1 Chain / AMPKg

Sujet: The 5-AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.Synonyms: 5'-AMP-activated protein kinase subunit gamma-1/2/3, AMPK gamma-1, AMPK gamma-2, AMPK gamma-3, AMPKG3, AMPKg, H91620p, PRKAG1, PRKAG2, PRKAG3

Poids moléculaire: approx. 40,42 kDa

ID gène: 5571

NCBI Accession: NP_002724

UniProt: P54619

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process, L'effet Warburg