Stathmin 1 anticorps (pSer16)

N° du produit ABIN498735

Cet anticorps anti-Stathmin 1 Polyclonal Lapin (ABIN498735) détecte spécifiquement Stathmin 1 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Stathmin 1 anticorps (pSer16) (ABIN498735)

Antigène: Stathmin 1 (STMN1)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Stathmin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Stathmin 1 anticorps

Épitope: pSer16

Specificité: This antibody detects endogenous levels of Op18 protein only when phosphorylated at Ser16.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Stathmin 1

Antigène: Stathmin 1 (STMN1)

Autre désignation: Stathmin / STMN1

Sujet: Oncoprotein 18 (Op18) is a regulator of microtubule (MT) dynamics. Op18 is a target for both cell cycle and cell surface receptor-coupled kinase systems, and phosphorylation of Op18 on specific combinations of amino acid residues has been shown to switch off MT-destabilizing activity of Op18. Induced expression of the catalytic subunit of cAMPdependent protein kinase (PKA) results in a dramatic increase in cellular MT polymer content concomitant with phosphorylation and partial degradation of Op18. Phosphorylation of Op18 on two specific sites, Serine 16 and Serine 63, is necessary and sufficient for PKA to switch off Op18 activity in intact cells. PKA phosphorylation down-regulates the MT-destabilizing activity of Op18, which in turn promotes increased Tubulin polymerization. Op18 has the potential to regulate the MT system in response to external signals such as cAMP-linked agonists.Synonyms: LAP18, Leukemia-associated phosphoprotein p18, Metablastin, OP18, Oncoprotein 18, Op18, Phosphoprotein p19, Prosolin, Protein Pr22, pp17, pp19

Poids moléculaire: approx. 19 kDa

ID gène: 3925

NCBI Accession: NP_005554

UniProt: P16949

Pathways: Signalisation MAPK, Dynamique des Microtubules