Connexin 43/GJA1 anticorps (pSer367)

N° du produit ABIN498754

Détail du produit anti-Connexin 43/GJA1 anticorps

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Épitope: pSer367

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Connexin 43/GJA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of Connexin 43 protein when phosphorylated at Ser367.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Connexin 43/GJA1

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Autre désignation: GJA1 / Cx43 (GJA1 Produits)

Synonymes: anticorps AVSD3, anticorps CX43, anticorps DFNB38, anticorps GJAL, anticorps HLHS1, anticorps HSS, anticorps ODDD, anticorps Cx43, anticorps AU042049, anticorps AW546267, anticorps Cnx43, anticorps Cx43alpha1, anticorps Gja-1, anticorps Npm1, anticorps connexin43, anticorps cx43a1, anticorps gja1, anticorps zfCx43.3, anticorps connexin 43, anticorps gja1-A, anticorps cx43, anticorps gjal, anticorps oddd, anticorps dfnb38, anticorps gap junction protein alpha 1, anticorps gap junction protein, alpha 1, anticorps connexin 43, anticorps gap junction protein alpha 1 L homeolog, anticorps CONNEXIN 43 protein, anticorps GJA1, anticorps Gja1, anticorps cx43, anticorps gja1.L, anticorps gja1, anticorps CONNEXIN 43

Sujet: The connexins are a group of GAP junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a GAP junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and GAP junction communication. There is a decrease in GAP junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of GAP junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases such as pp60v-Src and/or c-Src.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein

Poids moléculaire: approx. 43 kDa

ID gène: 2697

NCBI Accession: NP_000156

UniProt: P17302

Pathways: Signalisation MAPK, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization