ME1 anticorps

N° du produit ABIN499096

Cet anticorps Lapin Polyclonal détecte spécifiquement ME1 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ME1 anticorps (ABIN499096)

Antigène: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ME1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-ME1 anticorps

Specificité: This antibody detects endogenous levels of Malic enzyme 1 protein. (region surrounding Tyr500)

Réactivité croisée (Details): Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur ME1

Antigène: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Autre désignation: NADP-ME

Sujet: ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through its ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. The expression of ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell.Synonyms: ME1, NADP-dependent malic enzyme

Poids moléculaire: approx. 64 kDa

ID gène: 4199

NCBI Accession: NP_002386

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha