Phospholamban anticorps (N-Term)

N° du produit ABIN499141

Cet anticorps Chèvre Polyclonal détecte spécifiquement Phospholamban dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Rat, Boeuf (Vache) et Porc.

Aperçu rapide pour Phospholamban anticorps (N-Term) (ABIN499141)

Antigène: Phospholamban (PLN)

Reactivité: Humain, Rat, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Phospholamban est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Phospholamban anticorps

Épitope: N-Term

Séquence: KVQYLTRSAI RR-C

Specificité: Recognizes Phospholamban / PLN.

Réactivité croisée (Details): Species reactivity (expected):Pig, Bovine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the N Terminus of the protein sequence according to NP_002658.1. Genename: PLN

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.01-0.03 μg/mL. This antibody detects a band of approx. 13 kDa band in Human andRat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Phospholamban

Antigène: Phospholamban (PLN)

Autre désignation: Phospholamban

Sujet: Phospholamban (PLB/PLN) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains cAMP-dependent protein kinase phosphorylation sites and is highly basic and readily cleaved by proteases, whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. PLB is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.Synonyms: Cardiac phospholamban, PLB, PLN

ID gène: 5350

NCBI Accession: NP_002658

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity