POFUT1 anticorps (N-Term)

N° du produit ABIN500521

Cet anticorps Lapin Polyclonal détecte spécifiquement POFUT1 dans WB, IHC (p) et EIA. Il présente une réactivité envers Humain et Souris et a été mentionné dans 1 publication.

Aperçu rapide pour POFUT1 anticorps (N-Term) (ABIN500521)

Antigène: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POFUT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-POFUT1 anticorps

Épitope: N-Term

Specificité: This antibody detects POFUT1. It is predicted to not cross-react with POFUT2.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogène: POFUT1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human POFUT1.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: .5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-POFUT1 (ABIN500521)

Takeuchi, Wong, Schneider, Freeze, Takeuchi, Berardinelli, Ito, Lee, Nelson, Haltiwanger: "Variant in human POFUT1 reduces enzymatic activity and likely causes a recessive microcephaly, global developmental delay with cardiac and vascular features." dans: Glycobiology, Vol. 28, Issue 5, pp. 276-283, (2018) (PubMed).

Détails sur POFUT1

Antigène: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Autre désignation: POFUT1

Sujet: POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch, mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist.Synonyms: FUT12, GDP-fucose protein O-fucosyltransferase 1, KIAA0180, O-FucT-1, Peptide-O-fucosyltransferase 1

ID gène: 23509

NCBI Accession: NP_056167

UniProt: Q9H488

Pathways: Signalisation Notch, SARS-CoV-2 Protein Interactome