RNASEH2A anticorps (Center)

N° du produit ABIN500622

Cet anticorps anti-RNASEH2A est un anticorps Lapin Polyclonal détectant RNASEH2A dans WB et IF. Adapté pour Humain, Rat et Souris. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour RNASEH2A anticorps (Center) (ABIN500622)

Antigène: RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNASEH2A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-RNASEH2A anticorps

Épitope: Center

Specificité: This antibody detects RNAse H2A.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogène: RNAse H2A antibody was raised against a 17 amino acid peptide near the center of human RNAse H2A.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to 3 months, and at -20 °C thereafter.

Références pour anticorps anti-RNASEH2A (ABIN500622)

Zimmermann, Murina, Reijns, Agathanggelou, Challis, Tarnauskaitė, Muir, Fluteau, Aregger, McEwan, Yuan, Clarke, Lambros, Paneesha, Moss, Chandrashekhar, Angers, Moffat, Brunton, Hart, de Bono et al.: "CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. ..." dans: Nature, Vol. 559, Issue 7713, pp. 285-289, (2019) (PubMed).

Bartsch, Damme, Regen, Becker, Garrett, Hölter, Knittler, Borowski, Waisman, Glatzel, Fuchs, Gailus-Durner, Hrabe de Angelis, Rabe: "RNase H2 Loss in Murine Astrocytes Results in Cellular Defects Reminiscent of Nucleic Acid-Mediated Autoinflammation." dans: Frontiers in immunology, Vol. 9, pp. 587, (2018) (PubMed).

Cuadrado, Michailidou, van Bodegraven, Jansen, Sluijs, Geerts, Couraud, De Filippis, Vescovi, Kuijpers, Hol: "Phenotypic variation in Aicardi-Goutières syndrome explained by cell-specific IFN-stimulated gene response and cytokine release." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3623-33, (2015) (PubMed).

Détails sur RNASEH2A

Antigène: RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))

Autre désignation: RNASEH2A

Sujet: Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits, all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.Synonyms: AGS4, Aicardi-Goutieres syndrome 4 protein, RNASEHI, RNAse H2A, RNHIA, RNase H2 subunit A, RNase HI large subunit, Ribonuclease H2 subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A

ID gène: 10535

UniProt: O75792