DIABLO anticorps (C-Term)

N° du produit ABIN500784

Cet anticorps anti-DIABLO est un anticorps Lapin Polyclonal détectant DIABLO dans WB, IHC (p) et EIA. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour DIABLO anticorps (C-Term) (ABIN500784)

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DIABLO est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-DIABLO anticorps

Épitope: AA 222-237, C-Term

Specificité: This antibody detects DIABLO / SMAC at C-term.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogène: Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 0.5 to 1 μg/mL, 25 kDa band can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur DIABLO

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Autre désignation: DIABLO / SMAC

Sujet: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase

ID gène: 66593

UniProt: Q9JIQ3

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity