NSF anticorps (AA 151-250) (AbBy Fluor® 750)

N° du produit ABIN5007886

L’anticorps Lapin Polyclonal anti-NSF a été validé pour WB, IF (cc) et IF (p). Il convient pour détecter NSF dans des échantillons de Humain.

Aperçu rapide pour NSF anticorps (AA 151-250) (AbBy Fluor® 750) (ABIN5007886)

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSF est conjugé à/à la AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Détail du produit anti-NSF anticorps (AbBy Fluor® 750)

Épitope: AA 151-250

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human NSF

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur NSF

Antigène: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Autre désignation: NSF

Sujet:

Synonyms: N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor like protein, N ethylmaleimide sensitive fusion protein, N ethylmaleimide sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, NEM sensitive fusion protein, NEM sensitive fusion protein, NEM-sensitive fusion protein, NSF, NSF_HUMAN, SKD 2, SKD2, SKD2, Vesicle fusing ATPase, Vesicle fusing ATPase, Vesicle-fusing ATPase, Vesicular fusion protein NSF, Vesicular fusion protein NSF, Vesicular-fusion protein NSF.

Background: Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the -SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the -SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the -SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).