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Sonic Hedgehog anticorps (AA 237-399)

L’anticorps Lapin Polyclonal anti-Sonic Hedgehog a été validé pour WB, IHC, IP et ICC. Il convient pour détecter Sonic Hedgehog dans des échantillons de Rat.
N° du produit ABIN5013678

Aperçu rapide pour Sonic Hedgehog anticorps (AA 237-399) (ABIN5013678)

Antigène

Voir toutes Sonic Hedgehog (SHH) Anticorps
Sonic Hedgehog (SHH)

Reactivité

  • 73
  • 55
  • 37
  • 17
  • 10
  • 6
  • 6
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Hôte

  • 78
  • 16
  • 9
  • 1
  • 1
Lapin

Clonalité

  • 70
  • 35
Polyclonal

Conjugué

  • 60
  • 10
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Sonic Hedgehog est non-conjugé

Application

  • 78
  • 32
  • 29
  • 14
  • 13
  • 13
  • 11
  • 11
  • 10
  • 10
  • 5
  • 4
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Épitope

    • 15
    • 12
    • 9
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 237-399

    Fonction

    Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

    Specificité

    The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

     Réactivité croisée

    Humain, Souris

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    SHH (Ser237-Arg399)

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

    Agent conservateur

    Dithiothreitol (DTT), ProClin

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Antigène

    Sonic Hedgehog (SHH)

    Autre désignation

    Hedgehog Homolog, Sonic

    Sujet

    HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

    Pathways

    Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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