Topoisomerase II alpha anticorps (AA 1326-1512)

N° du produit ABIN5027278

L’anticorps anti-Topoisomerase II alpha Monoclonal Souris est utilisé pour la détection de Topoisomerase II alpha dans des échantillons de Humain. Il a été validé pour WB et FACS.

Aperçu rapide pour Topoisomerase II alpha anticorps (AA 1326-1512) (ABIN5027278)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: ABM48B4

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: AA 1326-1512

Purification: Protein G Chromatography

Immunogène: A partial length recombinant human Topo II alpha protein (amino acids 1326-1512) was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: WB: 1-2 μg/mL,FACS Analysis: 0.5-1 μg/10^6 cells

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: Topo II alpha

Sujet: Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response.

Poids moléculaire: 190 kDa

ID gène: 7153

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases