MED13 anticorps (AA 61-240)

N° du produit ABIN5519033

Cet anticorps anti-MED13 est un anticorps Lapin Polyclonal détectant MED13 dans ELISA, WB, FACS, ICC et IF. Adapté pour Humain et Souris.

Aperçu rapide pour MED13 anticorps (AA 61-240) (ABIN5519033)

Antigène: MED13 (Mediator Complex Subunit 13 (MED13))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MED13 est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-MED13 anticorps

Épitope: AA 61-240

Fonction: Anti-MED13 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-MED13 Antibody Picoband® (ABIN5519033). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human MED13 recombinant protein (Position: L61-Y240).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Gustafsson CM, Samuelsson T (Jul 2001). "Mediator--a universal complex in transcriptional regulation". Molecular Microbiology. 41 (1): 1-8. 2. Tuchin DA, Erve PR, Schumer W (1976). "Prevention of endotoxin--induced irreversible platelet aggregation in vitro". Surgical Forum. 27 (62): 22-3.

Commentaires:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur MED13

Antigène: MED13 (Mediator Complex Subunit 13 (MED13))

Autre désignation: MED13

Sujet:

Synonyms: Mediator of RNA polymerase II transcription subunit 13, Activator-recruited cofactor 250 kDa component, ARC250, Mediator complex subunit 13, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, Trap240, Vitamin D3 receptor-interacting protein complex component DRIP250, DRIP250, MED13, ARC250, KIAA0593, THRAP1, TRAP240,

Tissue Specificity: Ubiquitous.

Background: Mediator complex subunit 13 is a protein that in humans is encoded by the MED13 gene. This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Poids moléculaire: 300 kDa

ID gène: 9969

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha