POLI anticorps (C-Term)

N° du produit ABIN5531016

L’anticorps Lapin Polyclonal anti-POLI a été validé pour WB et IHC (p). Il convient pour détecter POLI dans des échantillons de Humain.

Aperçu rapide pour POLI anticorps (C-Term) (ABIN5531016)

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLI est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POLI anticorps

Épitope: AA 673-701, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This POLI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 673-701 amino acids from the C-terminal region of human POLI.

Isotype: Ig Fraction

Information d'application

Indications d'application: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur POLI

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Autre désignation: POLI

Sujet: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Poids moléculaire: 83 kDa

ID gène: 11201

UniProt: Q9UNA4

Pathways: Réparation de l'ADN