DKK4 anticorps (C-Term)

N° du produit ABIN5536032

Détail du produit anti-DKK4 anticorps

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Épitope: AA 188-217, C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DKK4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This DKK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the C-terminal region of human DKK4.

Isotype: Ig Fraction

Information d'application

Indications d'application: For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur DKK4

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Autre désignation: DKK4 (DKK4 Produits)

Synonymes: anticorps DKK-4, anticorps Dkk-4, anticorps RGD1563172, anticorps dickkopf WNT signaling pathway inhibitor 4, anticorps DKK4, anticorps Dkk4

Sujet: DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.

Poids moléculaire: 25 kDa

ID gène: 27121

UniProt: Q9UBT3

Pathways: Signalisation WNT