CD4 anticorps (PE)

N° du produit ABIN5541942

Il existe 2+ publications pour ce produit. L’anticorps anti-CD4 Monoclonal Souris est utilisé pour la détection de CD4 dans des échantillons de Cheval. Il a été validé pour FACS.

Aperçu rapide pour CD4 anticorps (PE) (ABIN5541942)

Antigène: CD4

Reactivité: Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: CVS4

Détail du produit anti-CD4 anticorps (PE)

Specificité: This anitbody CVS4 recognizes Equine CD4, a 58 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of equine T lymphocytes and considered to be the equine homologue of human CD4. A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).

Purification: Affinity Chromatography on Protein G

Immunogène: Equine thymocytes. Spleen cells from immunised mice were fused with cells of the X63-Ag 8.653 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry : Use 10 μL of neat antibody to label 10 6 cells in 100 μL.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-CD4 (PE) (ABIN5541942)

De Schauwer, Piepers, Van de Walle, Demeyere, Hoogewijs, Govaere, Braeckmans, Van Soom, Meyer: "In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry." dans: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 81, Issue 4, pp. 312-23, (2012) (PubMed).

Go, Zhang, Timoney, Cook, Horohov, Balasuriya: "Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes." dans: Journal of virology, Vol. 84, Issue 10, pp. 4898-911, (2010) (PubMed).

Détails sur CD4

Antigène: CD4

Autre désignation: cd4

Sujet: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

UniProt: F6Y6X8

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events