MCM2 anticorps

N° du produit ABIN5542011

L’anticorps anti-MCM2 Monoclonal Souris est utilisé pour la détection de MCM2 dans des échantillons de Humain. Il a été validé pour WB, IF et ICC.

Aperçu rapide pour MCM2 anticorps (ABIN5542011)

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCM2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 4B8

Détail du produit anti-MCM2 anticorps

Specificité: This antibody detects ~120 kDa of MCM2 protein from whole cell lysate of human cell lines.

Réactivité croisée (Details): Does not react with Mouse (WR19L) and Rat (Rat1).

Purification: Protein A agarose

Immunogène: Recombinant human MCM2 protein corresponding to full-length amino acids

Isotype: IgG1

Information d'application

Indications d'application: Western blot: 1 μg/mL for chemiluminescence detection system. Immunocytochemistry: 5-10 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Détails sur MCM2

Antigène: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Autre désignation: mcm2

Sujet: In human cells, MCM4, - 6, and - 7 proteins form a stable complex, and MCM2 is loosely associated with this complex. The heterotrimeric MCM complex containing MCM4, - 6 and - 7 ha s DNA helicase activity, and such activity can be inhibited by the presence of MCM2. Cdc7/Dbf4 protein kinase, which is required for the initiation of DNA replication phosphorylates MCM2 in vitro and in vivo. From these observations, it's thought that phos phorylation of MCM2 may remove MCM2 from the MCM4, - 6, and - 7 complex to activate its DNA helicase activity at the onset of DNA replication.

UniProt: P49736

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA