Recombinant KDM4A anticorps

N° du produit ABIN5563977

Détail du produit anti-KDM4A anticorps

Antigène: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KDM4A est non-conjugé

Application: Western Blotting (WB), ELISA

Marque: AbFlex®

Specificité: Mouse

Attributs du produit: AbFlex™ JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

Purification: Protein A Chromatography

Isotype: IgG2a

Information d'application

Indications d'application: WB: 1 - 2 μg/mL dilution Bead-based ELISA: 40 - 600 ng/mL

Commentaires:

AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur KDM4A

Antigène: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Autre désignation: JMJD2A (KDM4A Produits)

Synonymes: anticorps JMJD2A, anticorps JHDM3A, anticorps JMJD2, anticorps TDRD14A, anticorps D4Ertd222e, anticorps Jmjd2, anticorps Jmjd2a, anticorps mKIAA0677, anticorps lysine demethylase 4A, anticorps lysine-specific demethylase 4A, anticorps lysine (K)-specific demethylase 4A, anticorps KDM4A, anticorps LOC100228023, anticorps Kdm4a

Poids moléculaire: 130 kDa

Pathways: L'effet Warburg