TET1 anticorps (AA 1682-1914)

N° du produit ABIN5563987

Cité dans 2 publications. Cet anticorps anti-TET1 Monoclonal Rat (Clone 5D6) (ABIN5563987) détecte spécifiquement TET1 dans WB, IF, IP, ChIP-seq et ICC. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour TET1 anticorps (AA 1682-1914) (ABIN5563987)

Antigène: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TET1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Immunocytochemistry (ICC)

Clone: 5D6

Détail du produit anti-TET1 anticorps

Épitope: AA 1682-1914

Fonction: Tet1 antibody (mAb) (Clone 5D6)

Specificité: Mouse

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a recombinant protein corresponding amino acids 1682-1914 of mouse Tet1.

Isotype: IgG2a

Information d'application

Indications d'application: ChIP-Seq: 4 µL per ChIP This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91171.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-TET1 (ABIN5563987)

Thaler, Maurizi, Roschger, Sturmlechner, Khani, Spitzer, Rumpler, Zwerina, Karlic, Dudakovic, Klaushofer, Teti, Rucci, Varga, van Wijnen: "Anabolic and Antiresorptive Modulation of Bone Homeostasis by the Epigenetic Modulator Sulforaphane, a Naturally Occurring Isothiocyanate." dans: The Journal of biological chemistry, Vol. 291, Issue 13, pp. 6754-71, (2016) (PubMed).

Bauer, Göbel, Nagaraj, Colantuoni, Wang, Müller, Kremmer, Rottach, Leonhardt: "Phosphorylation of TET proteins is regulated via O-GlcNAcylation by the O-linked N-acetylglucosamine transferase (OGT)." dans: The Journal of biological chemistry, Vol. 290, Issue 8, pp. 4801-12, (2015) (PubMed).

Détails sur TET1

Antigène: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Autre désignation: TET1

Sujet: Tet1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.

Poids moléculaire: 215 kDa

NCBI Accession: NP_001240786

Pathways: Stem Cell Maintenance, L'effet Warburg