SNCA anticorps (Biotin)

N° du produit ABIN5564064

L’anticorps anti-SNCA Monoclonal Souris est utilisé pour la détection de SNCA dans des échantillons de Humain. Il a été validé pour WB, ELISA, IF, ICC et DB.

Aperçu rapide pour SNCA anticorps (Biotin) (ABIN5564064)

Antigène: SNCA (Synuclein, alpha (SNCA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SNCA est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 3C11

Détail du produit anti-SNCA anticorps (Biotin)

Specificité: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.,Binds within the 81-130 aa region of human ASYN, determined using WB and ELISA with corresponding peptides. Detects ~14 kDa band corresponding to ASYN. Band at ~30 kDa is a dimer.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Human alpha synuclein monomer

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• DB (1:1000)
• ICC/IF (1:100)
• ELISA (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN5564064 was sufficient for detection of Alpha Synuclein in 15 μg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur SNCA

Antigène: SNCA (Synuclein, alpha (SNCA))

Autre désignation: Alpha Synuclein

Sujet: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions, inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).

ID gène: 6622

NCBI Accession: NP_000336

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity