RASGRP2 anticorps (C-Term)

N° du produit ABIN5564284

Cet anticorps anti-RASGRP2 Polyclonal Lapin (ABIN5564284) détecte spécifiquement RASGRP2 dans WB. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour RASGRP2 anticorps (C-Term) (ABIN5564284)

Antigène: RASGRP2 (RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RASGRP2 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-RASGRP2 anticorps

Épitope: C-Term

Fonction: anti-RasGRP2, pAb (IG-09)

Specificité: Recognizes human and mouse RasGRP2.

Attributs du produit: Polyclonal Antibody. Recognizes human and mouse RasGRP2. Source: Rabbit. Applications: WB. Liquid. In PBS containing 2 mg/mL BSA and 0.05 % sodium azide. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.

Purification: Antigen affinity purified.

Pureté: Antigen affinity purified.

Immunogène: Synthetic peptide corresponding to a human RasGRP2 sequence located at the C-terminus of the RasGEF domain.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In PBS containing 2 mg/mL BSA and 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.Keep sterile.

Stock: 4 °C

Stockage commentaire:

+4°C

+4°C

Stable for at least 6 months after receipt when stored at +4°C.

Date de péremption: 6 months

Détails sur RASGRP2

Antigène: RASGRP2 (RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2))

Autre désignation: RasGRP2

Sujet:

Alternate Names/Synonyms: CalDAG-GEFI, Ras Guanyl Nucleotide-releasing Protein 2, Ras Guanyl Releasing Protein 2, CDC25-like Protein, CDC25L, Calcium and DAG-regulated Guanine Nucleotide Exchange Factor I, MCG7

Product Description: RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating small GTPases, including Rap1/RAS3, through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. The protein is expressed in platelets, neutrophils and T lymphocytes. RasGRP2 functions in aggregation of platelets and adhesion of T lymphocytes and neutrophils probably through inside-out integrin activation. S587 was identified as the major PKA phosphorylation. Phosphorylation of S587 correlated with the inhibitory effect of PKA on Rap1b activation in platelets. In vitro, ERK1/2 phosphorylates RasGRP2 at Ser394 to limit its exchange activity and inhibits its ability to activate the small GTPase Rap1 that ultimately leads to decreased activation of ERK1/2 in cells.

UniProt: Q7LDG7

Pathways: TCR Signaling